Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA Viral RNA. [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
Molnupiravir; molecular and functional descriptors of mitochondrial safety몰누피라비르; 미토콘드리아 안전성의 분자 및 기능 설명자Article Published on 2022-05-012022-09-11 Journal: Toxicology and applied pharmacology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료제, [키워드] active form Adult patients Analyzer antiviral drug antiviral drugs approved arrest cell line Concentration concentrations copy number COVID-19 Culture cytotoxicity DNA polymerase dose drug emergency treatment Endpoint expression flux functional Genome HepG2 HepG2 cell incorporation inhibit lack mechanism metabolite Mitochondria mitochondrial mitochondrial DNA Mitochondrial function mitochondrial gene molecular molnupiravir molnupiravir. Mutation N-hydroxycytidine NHC nucleoside Patient phenotypic PolG POLRMT progression RdRP replicating virus Replication resulting risk RNA polymerase significantly specificity the SARS-CoV-2 Toxicity Transcription Treatment triphosphate U.S. FDA viral genome virus was measured [DOI] 10.1016/j.taap.2022.116003 PMC 바로가기 [Article Type] Article
FV-100 for the Treatment of Varicella-Virus (VZV) Infections: Quo Vadis?Review Published on 2022-04-072023-05-31 Journal: Viruses [Category] 두창, 수두, 홍역, [키워드] Cf1743 DNA polymerase FV-100 herpes zoster thymidine kinase Varicella-zoster virus. [DOI] 10.3390/v14040770 PMC 바로가기 [Article Type] Review
Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman ProbeHFman 프로브를 사용한 SARS-CoV-2 검출을 위한 다중, 실시간, 현장 진료 RT-LAMPArticle Published on 2022-03-252022-09-11 Journal: ACS Sensors [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] addition affecting Alpha alpha and delta variants amplification arising binding Chain Reaction clinical sample COVID-19/SARS-CoV-2 Delta variants detection diagnostic diagnostic test DNA DNA polymerase Efficiency evade Evolution extracted RNA gold gold standard HFman probe high-fidelity DNA polymerase Impact isothermal LAMP limitation mechanism multiplex multiplex LAMP nasopharyngeal sample Nasopharyngeal samples nasopharyngeal swabs non-specific non-specific amplification nucleic acid overcome performed POCT point-of-care point-of-care testing (POCT). polymerase chain polymerase chain reaction positive primer probe Quantitative reaction Result reverse transcription RT-LAMP SARS-CoV-2 SARS-CoV-2 viral selective sensitivity sequence sequences specificity Spread subject Tolerance variant variants viral evolution [DOI] 10.1021/acssensors.1c02079 PMC 바로가기 [Article Type] Article
Polyphenols as Potential Inhibitors of SARS-CoV-2 RNA Dependent RNA Polymerase (RdRp)Article Published on 2021-12-082022-10-30 Journal: Molecules [Category] COVID-19, SARS, [키워드] Antiviral applied binding affinities candidate competing Compound computational approach COVID-19 demonstrated Dependent develop DNA DNA polymerase Drug discovery examined FDA-approved drug HIV inhibit inhibitory MD simulation mechanism molecular docking natural product polyphenol Potential RdRP Remdesivir RNA RNA polymerase SARS-CoV SARS-CoV-2 SARS-CoV-2 RNA transcriptase treatments for COVID-19 Virtual screening [DOI] 10.3390/molecules26247438 PMC 바로가기 [Article Type] Article
Structural basis for the interaction of SARS‐CoV ‐2 virulence factor nsp1 with DNA polymerase α–primaseSARS -COV -2 독성 인자 NSP1과 DNA 폴리머 라제 α -Primase의 상호 작용을위한 구조적 기초Full‐Length Paper Published on 2021-11-122022-09-10 Journal: Protein Science : A Publication of the Protein Soc [Category] COVID19(2023년), Fulltext, MERS, SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 binding biochemical biochemical evidence causative agent complex component coronavirus coronavirus disease Coronavirus disease 2019 cryo‐EM DNA DNA polymerase DNA polymerase α–primase genomic host protein human protein human proteins immune response Infection Interaction interfere Microscopy molecular mechanism molecular mechanisms nonstructural protein 1 nsp1 PDB polymerase prevented protein–protein interaction proteomic purified recent reported respiratory SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‐CoV‐2 scrutiny severe acute respiratory syndrome Coronavirus target the disease Viral viral infection virulence virulence factor virus [DOI] 10.1002/pro.4220 PMC 바로가기 [Article Type] Full‐Length Paper
Homebrew reagents for low-cost RT-LAMP저렴한 RT-LAMP용 자가제 시약Article Published on 2021-09-012022-09-11 Journal: Journal of biomolecular techniques : JBT [Category] SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 applicability buffer cold chain Concentration contribute coronavirus defined diagnostics diagnostics open source. distribution DNA DNA polymerase effort enzyme equipment house implementation in viral isothermal amplification limit limitation Local Loop-mediated isothermal amplification magnitude Murine leukemia virus Nasopharyngeal samples open popularity preparation protocol protocols reaction reagent reagents reduce reduction in Replication required respiratory reverse transcriptase reverse transcription RNA RT-LAMP RT-PCR SARS-CoV-2 sensitivity severe acute respiratory syndrome Coronavirus specificity Surveillance tested the preparation threshold transcriptase Viral [DOI] 10.7171/jbt.21-3203-006 PMC 바로가기 [Article Type] Article
Optimization and validation of RT-LAMP assay for diagnosis of SARS-CoV2 including the globally dominant Delta variant전 세계적으로 지배적 인 델타 변이체를 포함하여 SARS-COV2의 진단을위한 RT-LAMP 분석의 최적화 및 검증Research Published on 2021-08-302022-09-01 Journal: Virology Journal [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] acute respiratory syndrome coronavirus amplification Analysis B.1.1.7 B.1.1.7 (Alpha) B.1.351 B.1.351 (Beta) B.1.427 B.1.429 (Epsilon) B.1.525 B.1.525 (Eta) B.1.526 B.1.526 (Iota) B.1.617.1 B.1.617.1 (Kappa) B.1.617.2 B.1.617.2 (Delta) causative agent condition conserved conserved regions coronavirus coronavirus 2 COVID-19 COVID-19 pandemic delta variant development Diagnosis diagnosis of COVID-19 discrimination DNA polymerase dominant double blind double blind study evaluated gold gold standard Health Organization include infected individual infected individuals Isolation isothermal LAMP Lineage Loop-mediated isothermal amplification molecular Molecular diagnosis objective optimization P.1 P.1 (Gamma) P.2 P.2 (Zeta) P.3 (Theta) pathogenic coronaviruses Patient PCR primer binding site primer binding sites primer set Primers real time PCR Region respiratory Result RT-LAMP SARS-CoV-2 SARS-CoV2 severe acute respiratory syndrome coronavirus 2 Spread supplementary material Testing the disease time variants variants of concern variants of interest Visualization VoC WHO World Health Organization [DOI] 10.1186/s12985-021-01642-9 PMC 바로가기 [Article Type] Research
Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?루프 매개 등온 증폭(LAMP): PCR의 더 나은 형제?Review Published on 2021-07-292022-09-11 Journal: Cells [Category] SARS, 진단, 치료기술, 치료제, [키워드] amplified Analysis Better Chemical conducted denaturation diagnostic DNA DNA polymerase Effectiveness eight faster highlight incubator isothermal amplification Japanese LAMP LAMP method less Loop-mediated isothermal amplification naked eye PCR primer Primers RNA RT-LAMP SARS-CoV-2 SARS-CoV-2 detection sibling targets temperature [DOI] 10.3390/cells10081931 PMC 바로가기 [Article Type] Review
Hairpin-Spherical Nucleic Acids for Diagnosing COVID-19: a Simple Method to Generalize the Conventional PCR for Molecular AssaysCOVID-19 진단을 위한 머리핀-구형 핵산: 분자 분석을 위한 기존 PCR을 일반화하는 간단한 방법Article Published on 2021-07-062022-09-11 Journal: Analytical Chemistry [Category] SARS, 치료기술, 치료제, [키워드] absence acid activator acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Arm AuNP Chain Reaction clinical sample colorimetric detection conserved Conventional coronavirus COVID-19 COVID-19 pandemic detect develop diagnostic diagnostic testing Disease diagnosis DNA DNA polymerase E gene effort exonuclease Final gold Gold nanoparticle Infection Infectious disease Ligand loop modify nucleic acid nucleic acids pandemic Patient PCR PCR cycle PCR product polymerase chain polymerase chain reaction positive presence or absence probe Real-time reverse transcription PCR real-time RT-PCR Region Research reverse transcription PCR RNA RT-PCR SARS-CoV-2 SARS-CoV-2 RNA sensitivity sequence severe acute respiratory syndrome Coronavirus simple STEM TaqMan TaqMan probe [DOI] 10.1021/acs.analchem.1c01515 PMC 바로가기 [Article Type] Article